Figure 4

Validation of differentially expressed genes with quantitative real time PCR. QPCR was performed on RNA from samples used in the CodeLink analysis. Three selected metastatic RNA samples from each patient were pooled together (except for patient FB666 n = 1) and therefore four RNA samples, each representing one metastatic patient and 5 primary tumors were tested. The insert shows average expression values for metastatic and primary tumors.