Figure 2
From: Enhanced NFκB and AP-1 transcriptional activity associated with antiestrogen resistant breast cancer
Increased DNA-binding and transcriptional activity of AP-1 complexes expressed in TAM-resistant (MCF7/HER2, BT474) relative to TAM-sensitive (MCF7) breast cancer cells. A. DNA-binding by individual phospho-c-Jun and c-Fos subunits from nuclear extracts, quantitated by ELISA-based Trans-AM™ assays (mean ± SE OD450 nm values from replicate samples). B. (AP-1)-luciferase reporter gene expression (mean ± SE values from triplicate samples) measured after transient co-transfection of cells with the firefly reporter plasmid and a Renilla luciferase vector for normalization, as described in Methods. Asterisks (*) indicate significant difference (p < 0.05) from control MCF7 values.