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Figure 3 | BMC Cancer

Figure 3

From: Silencing of the XAF1 gene by promoter hypermethylation in cancer cells and reactivation to TRAIL-sensitization by IFN-β

Figure 3

Effect of IFN-β exposure on xaf1 expression at the transcript and protein levels. HeLa (A) and SK-N-AS (B) cells treated with 500 U/mL IFN-β for 0 hour, 24 hours, 48 hours or 5 days, or after a 7-day IFN-β free period were harvested, total RNA was extracted and subjected to quantitative RT-PCR and indexed to GAPDH transcript levels. (C) 30 μg of total protein extract were resolved by 12% SDS-PAGE and XAF1 was detected using an anti-XAF1 mouse monoclonal antibody. Loading was checked by using an anti-β-actin monoclonal antibody.

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