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Table 1 Methylation and expression of gene p16 INK4a in HR-HPV-positive cervical carcinomas and cervical cancer cell lines

From: Up-regulation of expression and lack of 5' CpG island hypermethylation of p16 INK4ain HPV-positive cervical carcinomas

Carcinoma/cells line Methylation status of 5' CpG island ** RT-PCR *** Immunochemical detection
    Number of stained nuclei (%) Cytoplasmic staining
1 1 - 0 Negative
2 3 + <1  
3 4 +   
4 0 + <5  
5 0 +   Positive in the majority of cancer cells
6 4 + <25  
7 5* +   
8 7 +   
9 12* +   
10 8* + >25  
11 3 +   
12 0 +   
13 10 + nt nt
C4-I 0 + <5  
HeLa 2 + 10–15 Positive in the majority of cells
SiHa 6 +   
C33A 0 +   
CaSki 0 + >25  
  1. * tumors that have methylated alleles, as determined by MSP
  2. ** number of partially methylated CpG dinucleotides out of 26 CpG dinucleotides in 5' region of p16 INK4a examined by bisulfite-modified DNA sequencing (see fig.1)
  3. *** more than 3 fold increase of the mRNA level in tumor compared to normal tissues