|
Carcinoma/cells line
|
Methylation status of 5' CpG island **
|
RT-PCR ***
|
Immunochemical detection
|
|---|
| | | |
Number of stained nuclei (%)
|
Cytoplasmic staining
|
|---|
|
1
|
1
|
-
|
0
|
Negative
|
|
2
|
3
|
+
|
<1
| |
|
3
|
4
|
+
| | |
|
4
|
0
|
+
|
<5
| |
|
5
|
0
|
+
| |
Positive in the majority of cancer cells
|
|
6
|
4
|
+
|
<25
| |
|
7
|
5*
|
+
| | |
|
8
|
7
|
+
| | |
|
9
|
12*
|
+
| | |
|
10
|
8*
|
+
|
>25
| |
|
11
|
3
|
+
| | |
|
12
|
0
|
+
| | |
|
13
|
10
|
+
|
nt
|
nt
|
|
C4-I
|
0
|
+
|
<5
| |
|
HeLa
|
2
|
+
|
10–15
|
Positive in the majority of cells
|
|
SiHa
|
6
|
+
| | |
|
C33A
|
0
|
+
| | |
|
CaSki
|
0
|
+
|
>25
| |
- * tumors that have methylated alleles, as determined by MSP
- ** number of partially methylated CpG dinucleotides out of 26 CpG dinucleotides in 5' region of p16 INK4a examined by bisulfite-modified DNA sequencing (see fig.1)
- *** more than 3 fold increase of the mRNA level in tumor compared to normal tissues
