(A). Dose-dependent growth inhibition of LCL8664 cells by TGF-β1. LCL8664 cells were cultured with TGF-β1 in varying concentrations from 0 ng/ml to 20 ng/ml. Viable cell counts were determined by trypan blue exclusion at regular intervals for 8 days. (B). Positive and negative growth regulation of LCL8664 cells by TGF-β1 and IL-6. LCL8664 cells were cultured with TGF-β1 (1 ng/ml), IL-6 (20 ng/ml), both, or were untreated (untr). Cell viability was quantified over 6 days of treatment using a tetrazolium dye reduction assay.