Table 1 Primers used in fragment analysis
Gene | Forward primer | Reverse primer | Microsatellite Repeat |
|---|---|---|---|
HLA A 4th ex | CCTGAATTTTCTGACTCTTCCCGT | GTTTCTTTCCCGCTGCCAGGTCAGTGT | 7(C) |
HLA A 5th ex | CCATCGTGGGCATCATTG | GTTTCTTTCAGTGAGACAAGAAATCTC | 3(GGA) |
HLA B 2nd ex | GCTTCATCTCAGTGGGCTAC | GTTTCTTCTCGCTCTGGTTGTA | 3(GA) + 3(CA) |
β2m 1st ex | GGCTGGGCACGCGTTTAAT | GTTTCTTAGGGAGAGAAGGACCAGAG | 4(CT) |
β2m 2nd ex (1) | TACCCTGGCAATATTAATGTG | GTTTCTTGATAGAAAGACCAGTCCTTGC | 4(GA) + 5(A) |
β2m 2nd ex (2) | CTTACTGAAGAATGGAGAGAG | GTTTCTTGACTACTCATACACAACTTTCA | 5(A) |
TAP1 1st ex | TAAATGGCTGAGCTTCTCGC | GTTTCTTAGAGCTAGCCATTGGCA | 5(C) |
TAP1 3rd ex | ACAGCCACTTGCAGGGAG | GTTTCTTTATGAACAGTACATGGCGTAT | 5(T) |
TAP1 8th ex | CTGCCCTGCTGCAGAATCTG | GTTTCTTCAAGCCACCTGCTTCCAT | 5(G) |
TAP1 10th ex | CTCTGCAGAGGTAGACGAGG | GTTTCTTATTAAGAAGATGACTGCCTCAC | 5(G) |
TAP1 11th ex | AGCACCTCAGCCTGGTGGA | GTTTCTTGCAGGTCTGAGAAGGCTTTC | 6(G) + 5(A) |
TAP2 2nd ex | TTCCTCAAGGGCTGCCAGGAC | GTTTCTTGCTCCAAGGGGCTGAAG | 6(C) |
TAP2 9th ex | CCTACGTCCTGGTGAGGTGA | GTTTCTTCTGGCTGTGCAGGTAGC | 5(G) |
Tapasin 2nd ex | TTGGTTCGTGGAGGATGC | GTTTCTTCCTAGAGACTCACCGTGTAC | 5(G) |
Tapasin 3rd ex | CTTCCTTCTCTACACTCAGACC | GTTTCTTAGGACTGGGCTGGATATGC | 5(C) |
Tapasin 4th ex | CCTGTCTTCCTCAGTGGTAC | GTTTCTTGAGCAGATGTCCCTTACCC | 6(C) |
Tapasin 5th ex | TGCTCATTTCGTCCTCTTTCC | GTTTCTTGTTCCCACTCCACCTCCAG | 5(G) |
Calnexin 7th ex | GAAGGATCAGTTCCATGACAAG | GTTTCTTCTGCATCTGGCCTCTTAGC | 5(A) |
Calnexin 8th ex | TCTGCTCAATGACATGACTCC | GTTTCTTTGAAGACAGTTCCCCAAGAC | 5(A) |
Calnexin 11th ex | AACCTTTCAGAATGACTCCTTTTAG | GTTTCTTCAAGCAGCAAACACGAACC | 8(T) |
Calreticulin 3rd ex | CTACCGTCCCGTCTCAGG | GTTTCTTTCTGTCTGGTCCAAACTATTAGG | 5(G) |
Calreticulin 6th ex | GACAAGCCCGAGCATATCC | GTTTCTTCACCTTGTACTCAGGGTTCTG | 5(C) |
ERp57 5th ex | CACTTATTGCTTCTTCCTTGTG | GTTTCTTAATACTTGGTCAGGAGATTCAAC | 6(T) |
ERp57 6th ex | CTTCTGCTATCTGCCTACTGAG | GTTTCTTTCAAGCAAATAAATCCCAGACAAG | 6(A) |
ERp57 13th ex | ACTTTTAAGCTGATCTTTCTGTTTT | GTTTCTTTTAGAGATCCTCCTGTGCCTT | 6(C) |
LMP2 2nd ex | GAGGGCATCAAGGCTGTTC | GTTTCTTGCAGACACTCGGGAATCAG | 5(G) |
LMP2 6th ex | CCCTCTCTCCAACTTGAAACC | GTTTCTTTGTAATAGTGACCAGGTAGATGAC | 5(G) |
LMP7 1st ex | GGCTTTCGCTTTCACTTCC | GTTTCTTGAGATCGCATAGAGAAACTGTAG | 6(C) |