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Figure 5 | BMC Cancer

Figure 5

From: E2F-1 induces melanoma cell apoptosis via PUMA up-regulation and Bax translocation

Figure 5

Bax mitochondrial translocation after Ad-E2F-1 expression in SK-MEL-2 cells and HCT116 PUMA+/+ cells. SK-MEL-2 cells (a,b) and HCT116PUMA+/+ cells (c,d) were infected with Ad-LacZ and Ad-E2F-1. After 24 hours of infection, cells were stained with Mitotracker Red 580 and Bax (green), then counterstained with DAPI (blue) as described in the Methods. After 24 hours of Ad-E2F-1 infection in SK-MEL-2 cells (b) and HCT116PUMA+/+ cells (d), Bax clustered and translocated to mitochondria (an overlay of the three stains is given in yellow, which shows co-localization of Bax in mitochondria).

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