Regulation of Bax and Bcl-2 protein expression on HepG2 cells by TGA. Cellular lysate protein (50 μg/lane) was loaded on a 10% SDS-polyacrylamide gel, electrophoresed, and subsequently transferred onto nitrocellulose. Immunoblots were detected with antibody specific for Bcl-2 and Bax. Lysates were from HepG2 cells treated with 50 μg/ml TGA for 0, 12, 24 and 48 h, respectively. The ratio of Bax/Bcl-2 was analyzed by pImage.