Table 1 Real-time PCR primers and probes used in this study
Target Gene | Gene Bank Acession NO. | Primer Sequence (5'-3') Sequences are shown for forward (F) and reverse (R) primers | Probe Sequence (5'-3')with modification of 5' FAM/3' BHQ1 | |
|---|---|---|---|---|
hRARα | NM_000964 | F | GACAAGTCCTCAGGCTACCACTATG | CTGCAAGGGCTTCTTCCGCCG |
R | GTACACCATGTTCTTCTGGATGCT | |||
hRARβ | NM_000965 | F | TCTCAGTGCCATCTGCTTAATCTG | CCAGGACCTTGAGGAACCGACAAAAG |
R | CCAGCAATGGTTCTTGTAGCTTATC | |||
hRARγ | NM_000966 | F | GCTGCAAGGGCTTCTTTCG | CGAAGCATCCAGAAGAACATGGTGTAC |
R | CAGTTTTTGTCGCGGTGACA | |||
hRXRα | NM_002957 | F | TCCTTGGAGGCCTACTGCAA | CAGCCGGGAAGGTTCGCTAAGC |
R | GCATTTGAGCCCGATGGA | |||
hRXRβ | NM_021976 | F | AGCAGCAGGGACGGTTTG | AAGCTGCTGCTACGTCTTCCTGCCC |
R | GCTCTAGACACTTAAGGCCAATGG | |||
hRXRγ | NM_006917 | F | ACCTTGGAGGACCAGGTCATT | TGCTTCGGGCAGGGTGGAATG |
R | GGAGAAAGAGGCAATCAGCAA | |||
hNur77 | NM_002135 | F | AGCATTATGGTGTCCGCACAT | TGAGGGCTGCAAGGGCTTCTTCAA |
R | TTGGCGTTTTTCTGCACTGT | |||
hNurr1 | NM_006186 | F | TGGGATGGTCAAAGAAGTGGTT | TTTAAAAGGCCGGAGAGGTCGTTTGC |
R | TGGGCTCTTCGGTTTCGA | |||
hNOR1 | NM_006981 | F | ATGCCCTTGTCCGAGCTTT | AACACCCAGAGATCTTGATTATTCCAGA |
R | AGCCTGGTCAGTGGGACAGT | |||
hCAR | NM_005122 | F | CACATGGGCACCATGTTTGA | TTTGTGCAGTTTAGGCCTCCAGCTCATCT |
R | AAGGGCTGGTGATGGATGAA | |||
SXR | NM_003889 | F | TCCCCAAATCTGCCGTGTAT | ACAAGGCCACTGGCTATCACTTCAATGTCA |
R | AGCCCTTGCATCCTTCACAT | |||
hPPARα | NM_005036 | F | AGCTCCCGTATCTTTTGTTATGTTG | GTCTGCGCTCCAGAGAGCATCTACTGTCA |
R | TCGATCCGCAGGGTGACT | |||
hβ-Actin | NM_001101 | F | CCTGGCACCCAGCACAAT | ATCAAGATCATTGCTCCTCCTGAGCGC |
R | GCCGATCCACACGGAGTACT |