Figure 3

RhoB expression was analyzed in various normal and cancer cell lines by RT-PCR. Expression was also analyzed in normal (N) and tumor (T) tissues obtained from patient operated from lung cancer. B. MSP analysis was performed on DNA extracted from the same cell lines and from normal and tumor tissues. U and M designed PCR product obtained with Unmethylated or Methylated specific primers. C. Scheme of the 5' RhoB promoter region. The 5' region of RhoB was identified previously [18]. We used a CpG island searcher [29] that screens for CpG islands that meet the following criteria: CG percentage > 55%; observed CpG/expected CpG > 0.65; length > 500 bp. This rich-CpG islands region is underlined, and all CpG are represented by vertical bars. D. An example of RhoB sequence after bisulfite sequencing is shown with CpG island in square. Comparison between normal and tumor tissue is shown.