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Figure 2 | BMC Cancer

Figure 2

From: Inhibition of PC cell-derived growth factor (PCDGF)/granulin-epithelin precursor (GEP) decreased cell proliferation and invasion through downregulation of cyclin D and CDK 4 and inactivation of MMP-2

Figure 2

PCDGF expression level after PCDGF antisense vector and control vectors transfection in SW626 cells. A, PCDGF mRNA expression levels were detected by RT-PCR after transfection of antisense PCDGF construct and three control groups including empty control, liposome control and control vector. The figure shows a representative result of three independent experiments. B, PCDGF protein expression levels were detected by western blot after transfection of antisense PCDGF construct and three control groups as above. C, quantitative detection of PCDGF mRNA level after PCDGF antisense vector and control vectors transfection in SW626 cells compared with GAPDH. Values represent the mean ± SE from three independent experiments. D, quantitative detection of PCDGF protein level after PCDGF antisense vector and control vectors transfection in SW626 cells compared with β-actin. Values represent the mean ± SE from three independent experiments.

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