Figure 7

TGZ changes the ratio of PTEN localized in cytosol and plasma membrane. (A) ES-2 cells were treated with various concentrations of TGZ for 24 h. Cell membrane and cytosolic fractions were isolated as described in Materials and Methods and then subjected to SDS-PAGE and Western blot analysis. The result of one representative assay from two similar independent experiments is shown. (B) Effect of TGZ on the distribution of PTEN. ES-2 cells were plated on FN (10 μg/mL)-coated coverslips and incubated in 10% FBS medium with 0.1% DMSO or 20 μM TGZ for 24 h. Cells were then double stained with paxillin (FITC) and PTEN (rhodamine). These results are representative of triplicate experiments.