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Figure 4 | BMC Cancer

Figure 4

From: Inhibition of cell motility by troglitazone in human ovarian carcinoma cell line

Figure 4

The TGZ-induced anti-migratory effect is independent of PPARγ expression. (A) ES-2 cells were either left untreated (UT) or transfected with PPARγ (Pγ) siRNA or control siRNA (50 nM each) as described in Materials and Methods, then treated with or without 20 μM TGZ for an additional 5 h. Western blot analysis of the cell lysates was carried out to determine the levels of PPARγ. Bottom of PPARγ panel indicates the quantity of PPARγ normalized to β-actin. All blots shown are one of three experiments. (B) Transwell assay of siRNA-transfected ES-2 cells. Mock or siRNA-transfected ES-2 cells were treated with TGZ for 5 h and cell migration quantified by Transwell assay as described in Figure 1 d. "Mock" indicates cells that were treated with transfection reagent. *P < 0.05 vs. DMSO-treated cells.

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