Figure 3
Inhibition of VEGF (A) and CA9 (B) mRNA expression under hypoxia: HT 1080 human fibrosarcoma cells were pre-treated with 150 nM chetomin (CHT) for four hours and transferred for 12 h to hypoxic (0.1% O2) or maintained in aerobic (20% O2) conditions. Quantitative real-time RT-PCR was performed to quantify the expression of VEGF or CA9 mRNA (n = 3, mean ± sem). Incubation with CHT caused a reduced hypoxic expression of CA9 and VEGF mRNA (* indicates significant difference from hypoxic control, p < 0.05).