Figure 1
Effect of chetomin on the fluorescence of EGFP under the control of a hypoxia-responsive promoter containing five copies of a hypoxia-responsive element (5HRE) or under the control of a hypoxia-independent constitutive CMV promoter (CMV) in stably transfected HT 1080 human fibrosarcoma cells. Cells were treated in air (20% O2) or hypoxia (0.1% O2). Black line indicates treatment with chetomin 150 nM, grey shaded area fluorescence of control cells. Chetomin has no effect on EGFP fluorescence in CMV cells, but reduces hypoxia-induced as well as basal normoxic EGFP fluorescence in 5HRE cells.