Effects of silencing Aurora-A/STK15 by RNA interference in HCT-STK15 and HCT-p53KO-STK15 cells. A) Western blot shows reduced expression of STK15 protein after 72 hours from siRNA transfection. B) FACScan analysis in HCT-STK15 and HCT-p53KO-STK15 cells left untreated and after 72 hours from siRNA transfection. DNA content was revealed by propidium iodide staining. HCT-STK15 cells show the presence of a sub-G1 peak indicating apoptosis in these cells. C) Histogram showing percentages of cells with the indicated centrosome numbers. D) Histogram showing percentages of aneuploid cells in both untransfected and silenced cells. Results in C and D were from three independent experiments (100–200 cells each) and bars indicate standard errors from the mean.