Figure 6

Repression of endogenous δEF1 abolishes BMP-6-induced transactivation of the E-cadherin promoter in MDA-MB-231 breast cancer cells. (a) δEF1-specific siRNA plasmid (siRNA-δEF1) was introduced into MDA-MB-231 cells to generate δEF1-interfered stable transfectants. Control cells were treated with a scrambled siRNA. The efficiency of δEF1 protein knockdown was examined by western blot, using an anti-ZEB antibody. Actin was used as a loading control. (b) δEF1-interfered MDA-MB-231 cell were transiently transfected with luciferase E-caherin promoter constructs. After transfection for 24 h, cells were treated with 200 ng/ml rhBMP-6. The luciferase activity of the extracts was determined 24 h after BMP-6 treatment using a Betascope analyzer. Luciferase values are normalized with Renilla activities. * indicates p < 0.05 in unpaired student t test when compared with vector alone.