Skip to main content
Figure 3 | BMC Cancer

Figure 3

From: BMP-6 promotes E-cadherin expression through repressing δEF1 in breast cancer cells

Figure 3

BMP-6 down-regulates δEF1 and concurrently up-regulates E-cadherin transcription in MDA-MB-231 breast cancer cells. (a) BMP-6-induced (200 ng/ml) down-regulation of δEF1 mRNA in MDA-MB-231 cells was verified by quantitative RT-PCR. GAPDH was used to normalize the δEF1 level. Data represent three independent experiments. (b) BMP-6-induced (200 ng/ml) up-regulation of E-cadherin mRNA in MDA-MB-231 cells was verified by quantitative RT-PCR. GAPDH was used to normalize the E-cadherin level. Data represent three independent experiments. (c) Western blot with anti-ZEB antibody showing δEF1 expression in MDA-MB-231 cells after culture for 24 or 48 h with BMP-6 (200 ng/ml). Actin expression was used as an internal control. (d) Western blot with anti-E-cadherin antibody showing E-cadherin expression in MDA-MB-231 cells after culture for 24 or 48 h with BMP-6 (200 ng/ml). Actin expression was used as an internal control.

Back to article page