Figure 1

Inhibition of human hepatocarcinoma cells growth by 5-Fu is due to increased apoptosis. (A) SMMC-7721 cells were treated with different concentrations of 5-Fu for 36 h, and cell viability was measured by MTT assay. (B) SMMC-7721 cells were incubated with (b,d) or without (a,c) 5-Fu (62.5 μg/ml) for 36 h were stained by the TUNEL method to reveal DNA strand breaks indicative of apoptosis. (a) (b) bright field micrographs corresponding to (c) and (d), respectively. (c)(d), micrographs taken with fluorescent microscopy (400×).