Figure 6

A. Cytochrome c release occurs between 8 and 24 after treatment. K562 cells were treated with both compounds and collected at each time point. The cytosolic and mitochondria-enriched fractions were prepared and western blot analyses were performed as described in Methods. B. K562/vector and K562/Bcl-2 were treated with PBTDs. The cytosolic and mitochondria-enriched fractions were prepared and western blot analyses were performed as described in Methods.