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Figure 5 | BMC Cancer

Figure 5

From: PYRROLO[1,2-b][1,2,5]BENZOTHIADIAZEPINES (PBTDs) induce apoptosis in K562 cells

Figure 5

A. Immunoblotting for caspase-3 (full-length and cleaved intermediate) using lysates from K562 cells treated with 10 μM PBTD-1 and PBTD-3 for the indicated time periods. The blots were stripped and reprobed with anti-actin antibody to ensure equal protein loading. Immunoblotting for each protein was done at least twice using independently prepared lysates, and the results were comparable. B. PARP cleavage was also analyzed by immunoblotting under the same condition Data indicate 116 kDa PARP (upper bands) and 85 kDa apoptosis-related cleavage fragment (lower bands). C. K562/vector and K562/Bcl-2 cells were treated with PBTDs. Equal amounts of cell lysates were subjected to electrophoresis by western blot for caspase-3.

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