Figure 5

Western Blot of nuclear and cytosolic fractions of the cancer cell lines MCF-7, BT-20, MDA-MB231 and ZR-75/1. LASP-1 is detectable in nuclear (N) as well as in cytoplasmatic (C) cell fractions of breast cell lines MCF-7 and BT-20, but not in the nucleus of MDA-MB231 and ZR-75/1 cells. GAPDH was used as a cytoplasmatic marker, Lamin A + C as nuclear markers to exclude contamination during cell fraction isolation.