Table 1 Primers used for mutation analysis, sequencing, cloning and mutagenesis of mouse Bcl11b.
Fragment | Direction | Sequence (5'->3') | Size of PCR product (bp) | Annealing temp. (°C)/Cycles |
|---|---|---|---|---|
ex1 | F | CGC ATC TGT GCA GCT TTC | 226 | 56/35 |
R | CCG GCT GCA GAG AAA CTT | |||
ex2a | F | TGT CTT CTC TGC CCC TTC C | 264 | 59/35 |
R | CTT GTC CAG GAC CTT GTC GT | |||
ex2b | F | ACC TGT GGC CAG TGT CAG AT | 300 | 59/35 |
R | TAG GGA GGC AGC TTC AGC A | |||
ex3 | F | GGC ACC ACT GAC CAG TCT TT | 377 | 61/35 |
R | CTT TTT GAG TGG GGG ATG G | |||
zfd1 | F | TGC TCA CAC TCT GCC TTT CTT | 291 | 59/35 |
R | GCA GCA GGT TGA AAG GAT TG | |||
ex4a | F | TGA ATT TCC TGG GGG ACA G | 283 | 60/35 |
R | CTG TTG CCG GCC AGT TCT | |||
prd | F | TCT CCT GCC ATG GAC TTC TC | 329 | 62/35 |
R | TGG CAC TTG TAG GGC TTC TC | |||
zfd2 | F | AGT CCC AAG TCC CCG TTC | 299 | 60/35 |
R | CAG CTG AGA GCC CGT CGT | |||
acd | F | ATG AAG ACG CAC ATG CAC AA | 289 | 64/35 |
R | CAC GCC ACC TCC GTT CTC | |||
ex4b | F | AGC ATG GAC TCG GAG CTG | 300 | 62/35 |
R | AGC TGG AAA GGG CTC TGC | |||
ex4c | F | GTG ATG CCG GAG CTG TTG | 291 | 59/35 |
R | CCA GGA ATG GGT CCT TCA | |||
ex4d | F | GTG TAC TCG CAG TGG CTC GT | 294 | 60/35 |
R | GAC CTT GCC GCA GTA CTC AC | |||
zfd3 | F | ACA CCT CAC CTG GGT GGT C | 274 | 59/35 |
R | TCA TTA GTC AGC AAG TGT TCA CC | |||
cloning | F | TTT GAA TTC ATG TCC CGC CGC AAA C | 2459 | 65/35 |
R | TTT GCG GCC GCT TAG CTC CTC TCA GCC TGC TC | |||
S778N | F | CTG GGA GGC CGA ACT CCA AGG AGG GC | 65/12 | |
R | GCC CTC CTT GGA GTT CGG CCT CCC AG | |||
K828T | F | GCA GAG CAG CAC GCT CAC GCG CCA C | 65/12 | |
R | GTG GCG CGT GAG CGT GCT GCT CTG C | |||
Y844C | F | GCA AGG AGG TGT GCC GCT GCG ACA TC | 65/12 | |
R | GAT GTC GCA GCG GCA CAC CTC CTT GC | |||
FS823 | F | CGA GCT GTG CAA CTA CGC GCT AAG AGC AGC AAG CTC ACG CG | 65/18 | |
R | CGC GTG AGC TTG CTG CTC TTA GCG CGT AGT TGC ACA GCT CG |