Expression of NDRG2 is down-regulated in high-risk adenomas and colorectal carcinoma

BMC Cancer

Table 2 Expression analysis of NDRG2 in colon cancer using a Cancer Profiling Array.

Sample	Signal intensity	Tumor/normal	TNM staging	Age	Gender
Tumor/normal	18370/60015	0.31	II; T4N0M0	67	Female
Tumor/normal	31402/35431	0.89	I; T1N0M0	58	Female
Tumor/normal	57125/96121	0.59	IV; T4N0M0	43	Female
Tumor/normal	20382/23650	0.86	IIIB; T3N1M0	69	Female
Tumor/normal	28165/59167	0.48	IIIB; T3N1M0	35	Female
Tumor/normal	22193/12912	1.72	IIIA; T4N0M0	58	Male
Tumor/normal	18569/28122	0.66	IIIB; T4N1M0	63	Male
Tumor/normal	9863/28914	0.34	IIA; T2N0M0	73	Female
Tumor/normal	21780/34774	0.63	IIIB; T4N0M?	65	Female
Tumor/normal	22429/45939	0.49	IIIA; T3N1M0	65	Female

Expression of NDRG2 mRNA was determined by hybridisation of a Cancer Profiling Array with a NDRG2 probe. Quantification of the hybridisation signal for the paired samples revealed that NDRG2 expression was reduced in 9 out of 10 colon tumor samples compared to the corresponding normal sample. Using a two-tailed paired t-test, the decrease in NDRG2 expression in tumors compared to normal samples was found to be statistically significant (p < 0.01). The tumors were all diagnosed as adenocarcinoma aside from the Stage I tumor which was a tubulovillous adenoma. The TNM staging system describes the extent of the primary tumor (T), the absence/presence of metastasis to nearby lymph nodes (N) and the absence/presence of distant metastases (M).

ISSN: 1471-2407