NDRG2 mRNA levels are down-regulated during colorectal cancer carcinogenesis. mRNA expression of NDRG2 determined by real-time RT-PCR and normalised to β-actin in healthy individuals (Control), normal and affected tissue from the same individual with adenomas (low- or high-risk) and colorectal carcinoma. Normal (adjacent): normal sample close to the carcinoma, Normal (distant): normal sample far from the carcinoma. Each dot represents mean values of triplicate determinations. *** p < 0.001 compared to the control group using one-way ANOVA with a Tukey's post test. A trend of decreased NDRG2 expression with increasing tumor grade was observed in affected tissue (p < 0.001).