Table 2 Proteins with differential expression between GCA tumour tissues and adjacent paired non-tumour tissues were identified by MALDI-TOF MS
From: Proteome analysis of human gastric cardia adenocarcinoma by laser capture microdissection
Up-regulation proteins | |||||||
|---|---|---|---|---|---|---|---|
Spot No. | Protein | Accession No.a) | Mr/pIb) | Match | Cov (%)c) | T-test | Ration (tumour/non-tumour) Means ± SD |
1 | Heat-shock protein beta-1(HSP27)* | P04792 | 23/6.0 | 9 | 54 | 0.0040 | 3.4279 ± 2.0727 |
2 | Heat shock 70 kDa protein 5(HSP70) | P11021 | 70/5.0 | 22 | 42 | 0.0030 | 3.2597 ± 1.5314 |
3 | 60 kDa heat shock protein(HSP60)* | P10809 | 58/5.2 | 12 | 37 | 0.0001 | 2.8308 ± 1.1426 |
4 | Protein disulfide isomerase A3(PDIA3)* | P30101 | 54/5.6 | 13 | 41 | 0.0280 | 4.2105 ± 2.7294 |
5 | Annexin A4(ANXA4) | P09525 | 36/5.8 | 10 | 34 | 0.0140 | 4.7153 ± 7.3434 |
6 | Annexin A2(ANXA2) | P07335 | 38/7.5 | 7 | 26 | 0.0150 | 6.0722 ± 5.5431 |
7 | Heterogeneous nuclear ribonucleoproteins A2/B1(hnRNP A2/B1)* | P22626 | 37/9.0 | 16 | 43 | 0.0050 | 10.7775 ± 9.5547 |
8 | Cathepsin D heavy chain(CTSD) | P07339 | 27/5.6 | 12 | 61 | 0.0012 | 4.3552 ± 3.7703 |
9 | Protein kinase C and casein kinase substrate in neurons protein 1 (PACSIN1) | Q9BY11 | 51/5.1 | 5 | 24 | 0.0028 | 2.6026 ± 1.2147 |
10 | Glutathione S- transferase P(GSTP)* | P09211 | 23/5.4 | 5 | 44 | 0.0026 | 3.8757 ± 2.2198 |
11 | Peroxiredoxin-1(Prx-1)* | Q06830 | 22/8.3 | 8 | 59 | 0.0140 | 4.2744 ± 4.1568 |
12 | Poly(rC)-binding protein1(PCBP1)* | Q15360 | 37/6.7 | 12 | 63 | 0.0070 | 2.7401 ± 0.5321 |
13 | Aldo-keto reductase family 1member C3 (AKR1C3) | P42330 | 37/8.1 | 7 | 25 | 0.0040 | 2.7762 ± 1.1820 |
14 | Glycineamidinotransferase (GATM) | P50440 | 44/6.4 | 8 | 23 | 0.0390 | 2.7839 ± 0.4680 |
15 | Keratin, type II cytoskeletal 8 (Keratin 8)* | P05787 | 54/5.5 | 9 | 25 | 0.0020 | 3.5063 ± 0.6423 |
Down-regulation proteins | |||||||
16 | Actin, cytoplasmic 1 (Actin 1)* | P60709 | 42/5.3 | 6 | 30 | 0.0160 | 0.2603 ± 0.1539 |
17 | Heterogeneous nuclear ribonucleoproteins H3(hnRNPH3) | P31942 | 37/6.4 | 5 | 28 | 0.0015 | 0.6028 ± 0.6229 |
18 | Peroxiredoxin-2(Prx-2)* | P32119 | 22/5.7 | 6 | 24 | 0.0250 | 0.2376 ± 0.1202 |
19 | Carbonic anhydrase2(CA2) * | P00918 | 29/6.8 | 6 | 33 | 0.0130 | 0.3675 ± 0.1270 |
20 | Alpha-enolase*(ENO1) | P06733 | 47/7.0 | 16 | 42 | 0.0030 | 0.2898 ± 0.1340 |
21 | Alcohol dehydrogenase 1C (ADH1C) | P00326 | 40/8.6 | 7 | 49 | 0.0280 | 0.2287 ± 0.1680 |
22 | Electron transfer flavoprotein subunit b (ETFB) | P38117 | 28/8.3 | 6 | 26 | 0.0240 | 0.2655 ± 0.1415 |
23 | Voltage-dependent anion-selective channel (VDAC)* | P21796 | 31/8.6 | 7 | 39 | 0.0120 | 0.4474 ± 0.0297 |