Table 1 RAPD primer sequences and PCR conditions
From: Clonal mutations in primary human glial tumors: evidence in support of the mutator hypothesis
Primer ID | Primer Sequence | Magnesium Chloride Concentration (mM) | Annealing Temperature (°C) |
|---|---|---|---|
50/09 | 5' AGAAGCGATG 3' | 1.5 | 28 |
50/24 | 5' GTTAGTGGCA 3' | 3.0 | 22 |
60/39 | 5' CGCTGTTACC 3' | 3.0 | 28 |
70/09 | 5' TGCAGCACCG 3' | 3.0 | 42 |
70/31 | 5' GCCCCTCTT G 3' | 3.0 | 36 |
70/34 | 5' GGACCGCTAG 3' | 3.0 | 33 |
70/40 | 5' CGCAGACCTC 3' | 4.5 | 33 |
80/03 | 5' CCATGGCGCC 3' | 3.0 | 48 |
80/06 | 5' GCACGGAGGG 3' | 4.5 | 42 |
80/32 | 5'GCCCCATGCG 3' | 4.5 | 48 |
80/35 | 5' CACCTGCCGC 3' | 1.5 | 45 |
80/37 | 5' CGCCAGGAGC 3' | 4.5 | 42 |
RE-05 | 5' GCGAATTCCG 3' | 3.0 | 37 |
RE-06 | 5' CGGAATTCCG 3' | 3.0 | 37 |
RE-21 | 5' GGCTGCAGCG 3' | 3.0 | 45 |