Effects of LM-234ep conditioned medium on cell cycle and its regulatory proteins. B16-F10 and HeLa cells were treated with culture medium (Control) or LM-234ep CM for 48 h. Cell cycle progression was analyzed by flow cytometry (A). After the treatment for the indicated times (top) with LM-234ep CM or culture medium (Control), B16-F10 (B) and HeLa (D) cells were collected, lysed, and analyzed by immunoblotting using antibodies specific for cyclins E, A, and D, and Cdk2. β-actin was used as a loading control. Immunofluorescence for Cyclin A1 in B16-F10 (C), and HeLa (E) cells after 24 h incubation with culture medium or LM-234ep CM. Magnification bar, 40 μm.