Effect of various inhibitors on the DFO-induced IL-8 mRNA expression. Cells were incubated for 16 h in the presence or absence of DFO 1.0 mM with and without GSNO (1.0 mM), PDTC (50 μM), hemoglobin (1 mg), wortmannin (0.5 μM), and cycloheximede (10 μg/ml). Same procedure as described in the legend to Fig. 1 was performed. The results are representative of three independent experiments.