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Figure 4 | BMC Cancer

Figure 4

From: Differential regulation of iron chelator-induced IL-8 synthesis via MAP kinase and NF-κB in immortalized and malignant oral keratinocytes

Figure 4

DFO induced the DNA binding activities of NF-kB in IHOK and HN12 cells. The nuclear extracts from the cells treated with DFO 1.0 mM at the indicated times were incubated with [γ-32P] ATP-labeled NF-kB probe, and analyzed by EMSA. The specificity of the bands was verified by adding a 10-fold excess of a completing unlabeled NF-kB probe (cold probe) to the 4 h DFO treated unclear proteins. The results were confirmed by two independent experiments.

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