Table 2 Primers and PCR programs for CTLA-4 PCR-RFLP genotyping
From: Association of CTLA-4 gene polymorphisms with sporadic breast cancer in Chinese Han population
Reference SNP ID | Gene/SNP primer | Sequence | PCR program |
|---|---|---|---|
rs4553808 | CTLA-4 -1661G/A F | 5'-CTAAGAGCATCCGCTTGCACCT-3' | 94°C 5 min; 30 cycles, 94°C 15s, |
CTLA-4 -1661G/A R | 5'-TTGGTGTGATGCACAGAAGCCTTT-3' | 58°C 5s, 72°C 45s; 72°C 7 min | |
rs11571317 | CTLA-4 -658T/C F | 5'-ATCATTGGTCCTGTCTACAGC-3' | 94°C 5 min; 35 cycles, 94°C 30s, |
CTLA-4 -658T/C R | 5'-CTTCTAATGGTCCCTTGACAG-3' | 55.5°C 10s, 72°C 30s; 72°C 5 min | |
rs5742909 | CTLA-4 -318T/C F | 5'-AAATGAATTGGACTGGATGGT-3' | 94°C 4 min; 30 cycles, 94 °C 30s, |
CTLA-4 -318T/C R | 5'-TTACGAGAAAGGAAGCCGTG-3' | 55.6°C 5s, 72°C 30s; 72°C 4 min | |
rs231775 | CTLA-4 +49G/A F | 5'-AGTCTCACTCACCTTTGCAG-3' | 94°C 4 min; 30 cycles, 94°C 30s, |
CTLA-4 +49G/A R | 5'-GCTCTACCTCTTGAAGACCT-3' | 56.4°C 10s, 72°C 30s; 72°C 4 min | |
rs3087243 | CTLA-4 CT60G/A F | 5'-GAGGTGAAGAACCTGTGTGTTAAA-3' | 94°C 5 min; 30 cycles, 94°C 30s, |
CTLA-4 CT60G/A R | 5'-ATAATGCTTCATGAGTCAGCTT-3' | 55.6°C 30s, 72°C 30s; 72°C 5 min |