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Table 2 Primers and PCR programs for CTLA-4 PCR-RFLP genotyping

From: Association of CTLA-4 gene polymorphisms with sporadic breast cancer in Chinese Han population

Reference SNP ID Gene/SNP primer Sequence PCR program
rs4553808 CTLA-4 -1661G/A F 5'-CTAAGAGCATCCGCTTGCACCT-3' 94°C 5 min; 30 cycles, 94°C 15s,
  CTLA-4 -1661G/A R 5'-TTGGTGTGATGCACAGAAGCCTTT-3' 58°C 5s, 72°C 45s; 72°C 7 min
rs11571317 CTLA-4 -658T/C F 5'-ATCATTGGTCCTGTCTACAGC-3' 94°C 5 min; 35 cycles, 94°C 30s,
  CTLA-4 -658T/C R 5'-CTTCTAATGGTCCCTTGACAG-3' 55.5°C 10s, 72°C 30s; 72°C 5 min
rs5742909 CTLA-4 -318T/C F 5'-AAATGAATTGGACTGGATGGT-3' 94°C 4 min; 30 cycles, 94 °C 30s,
  CTLA-4 -318T/C R 5'-TTACGAGAAAGGAAGCCGTG-3' 55.6°C 5s, 72°C 30s; 72°C 4 min
rs231775 CTLA-4 +49G/A F 5'-AGTCTCACTCACCTTTGCAG-3' 94°C 4 min; 30 cycles, 94°C 30s,
  CTLA-4 +49G/A R 5'-GCTCTACCTCTTGAAGACCT-3' 56.4°C 10s, 72°C 30s; 72°C 4 min
rs3087243 CTLA-4 CT60G/A F 5'-GAGGTGAAGAACCTGTGTGTTAAA-3' 94°C 5 min; 30 cycles, 94°C 30s,
  CTLA-4 CT60G/A R 5'-ATAATGCTTCATGAGTCAGCTT-3' 55.6°C 30s, 72°C 30s; 72°C 5 min