RT-PCR analysis of the fra-1 gene expression in paraffin-embedded tissues. The mRNA was extracted from paraffin-embedded tissues and amplified by RT-PCR using fra-1 specific primers as specified under "Material and Methods". The cDNAs were co-amplified with gapdh gene as an internal control. Sources of RNA: lane 1, normal breast tissue; lanes 2–4, breast dysplastic lesions; lanes 5–7, breast fibroadenomas; lanes 8–12, breast carcinomas; lane 13: water control. Molecular markers are indicated.