Figure 3
From: Over-expression of 14-3-3zeta is an early event in oral cancer
Immunoblot analysis of 14-3-3ζ in oral tissues. Equal amounts of whole cell lysates of normal oral tissues (N), OPLs (P) and OSCCs (C) from the same patients as used for IHC were resolved on 12% SDS-PAGE. Proteins were then electro-transferred on PVDF membrane followed by blocking with 5% non-fat milk O/N. Blots were incubated with anti-14-3-3ζ polyclonal antibody and protein expression was determined using Enhanced chemiluminescence method. Immunoblots of: (a) 14-3-3ζ protein and (b) α-tubulin, in normal oral tissue (N), oral pre-malignant lesion (P) and OSCC (C).