Figure 1

Immunohistochemical analysis of 14-3-3ζ in oral tissues. Paraffin embedded oral tissue sections were stained using anti-14-3-3ζ antibody as described in Materials and Method section. (a) Normal oral mucosa showing no detectable immunostaining of 14-3-3ζ protein (original magnification ×100). (b) Inflamed normal oral mucosa showing cytoplasmic immunostaining of 14-3-3ζ protein (original magnification × 100). (c) Oral hyperplasia showing cytoplasmic and nuclear immunostaining of 14-3-3ζ(original magnification × 100). (d) Oral hyperplasia showing nuclear and cytoplasmic immunostaining at higher magnification (original magnification × 400). (e) Dysplastic lesion showing cytoplasmic immunostaining in epithelial cells (original magnification × 200). (f) OSCC showing cytoplasmic and nuclear staining in tumor cells (original magnification × 100). (g) OSCC showing cytoplasmic and nuclear staining in tumor cells (original magnification × 400). (h) OSCC showing cytoplasmic and membranous staining in tumor cells (original magnification × 400). (i) Negative control showing no immunostaining of 14-3-3ζ protein in epithelial cells (original magnification × 100). (j) Positive control is a known esophageal squamous cell carcinoma showing cytoplasmic immunostaining of 14-3-3ζ in epithelial cells (original magnification × 100). Arrow shows nuclear staining in Panels 1d, 1e and 1 h and membranous staining in Panel 1 g respectively.