From: High resolution melting for mutation scanning of TP53exons 5–8
Exon | Primer name | Sequence | Annealing conditions |
---|---|---|---|
5a | TP53_Exon5a_F | CAACTCTGTCTCCTTCCTCTTCCTAC | 65–60°C touchdown 0.5°C/cycle for 10 cycles |
TP53_Exon5a_R | AGCCATGGCACGGACGCG | ||
5b | TP53_Exon5b_F | CTCCTGCCCGGCACCCGC | 65–60°C touchdown 0.5°C/cycle for 10 cycles |
TP53_Exon5b_R | CTAAGAGCAATCAGTGAGGAATCAGA | ||
6 | TP53_Exon6_F | CAACCACCCTTAACCCCTCCT | 68–58°C touchdown 1.0°C/cycle for 10 cycles |
TP53_Exon6_R | AGACGACAGGGCTGGTTGC | ||
7 | TP53_Exon7_F | AGGCGCACTGGCCTCATC | 68–58°C touchdown 1.0°C/cycle for 10 cycles |
TP53_Exon7_R | GAGGCTGGGGCACAGCA | ||
8 | TP53_Exon8_F | GACCTGATTTCCTTACTGCCTCTTG | 63.5–58.5°C touchdown 0.5°C/cycle for 10 cycles |
TP53_Exon8_R | AATCTGAGGCATAACTGCACCCTT |