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Table 1 TP53 HRM primers and PCR annealing temperature conditions

From: High resolution melting for mutation scanning of TP53exons 5–8

Exon

Primer name

Sequence

Annealing conditions

5a

TP53_Exon5a_F

CAACTCTGTCTCCTTCCTCTTCCTAC

65–60°C touchdown 0.5°C/cycle for 10 cycles

 

TP53_Exon5a_R

AGCCATGGCACGGACGCG

 

5b

TP53_Exon5b_F

CTCCTGCCCGGCACCCGC

65–60°C touchdown 0.5°C/cycle for 10 cycles

 

TP53_Exon5b_R

CTAAGAGCAATCAGTGAGGAATCAGA

 

6

TP53_Exon6_F

CAACCACCCTTAACCCCTCCT

68–58°C touchdown 1.0°C/cycle for 10 cycles

 

TP53_Exon6_R

AGACGACAGGGCTGGTTGC

 

7

TP53_Exon7_F

AGGCGCACTGGCCTCATC

68–58°C touchdown 1.0°C/cycle for 10 cycles

 

TP53_Exon7_R

GAGGCTGGGGCACAGCA

 

8

TP53_Exon8_F

GACCTGATTTCCTTACTGCCTCTTG

63.5–58.5°C touchdown 0.5°C/cycle for 10 cycles

 

TP53_Exon8_R

AATCTGAGGCATAACTGCACCCTT

 
  1. Underlined nucleotides are introduced sequence