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Table 2 Details of PCR conditions for polymorphism analysis

From: Polymorphisms in XPC, XPD, XRCC1, and XRCC3 DNA repair genes and lung cancer risk in a population of Northern Spain

Product Primer sequence Size (bp) PCR Conditions
XPC (PAT) (F) TAG CAC CCA GCA GTC AAA G
(R) TGT GAA TGT GCT TAA TGC TG
266/344 30 cycles: 94°C 30s, 58°C 30s, 72°C 30s
XPD exon 10 (F) CTG TTG GTG GGT GCC CGT ATC TGT TGG TCT
(R) TAA TAT CGG GGC TCA CCC TGC AGC ACT TCC T
751 40 cycles: 94°C 30s, 65°C 30s, 72°C 1 min
XPD exon 23 (F) GCC CGC TCT GGA TTA TAC G
(R) CTA TCA TCT CCT GGC CCC C
436 30 cycles: 94°C 30s, 64°C 30s, 72°C 45s
XRCC1 (F) CAG TGG TGC TAA CCT AAT C
(R) AGT AGT CTG CTG GCT CTG G
871 30 cycles: 94°C 30s, 64°C 30s, 72°C 1 min
XRCC3 (F) GCC TGG TGG TCA TCG ACT C
(R) CAG GGC TCT GGA AGG CAC TGC TCA GCT CAC GCA cC
136 30 cycles: 94°C 30s, 65°C 30s, 72°C 1 min