Table 2 Details of PCR conditions for polymorphism analysis
Product | Primer sequence | Size (bp) | PCR Conditions |
|---|---|---|---|
XPC (PAT) | (F) TAG CAC CCA GCA GTC AAA G (R) TGT GAA TGT GCT TAA TGC TG | 266/344 | 30 cycles: 94°C 30s, 58°C 30s, 72°C 30s |
XPD exon 10 | (F) CTG TTG GTG GGT GCC CGT ATC TGT TGG TCT (R) TAA TAT CGG GGC TCA CCC TGC AGC ACT TCC T | 751 | 40 cycles: 94°C 30s, 65°C 30s, 72°C 1 min |
XPD exon 23 | (F) GCC CGC TCT GGA TTA TAC G (R) CTA TCA TCT CCT GGC CCC C | 436 | 30 cycles: 94°C 30s, 64°C 30s, 72°C 45s |
XRCC1 | (F) CAG TGG TGC TAA CCT AAT C (R) AGT AGT CTG CTG GCT CTG G | 871 | 30 cycles: 94°C 30s, 64°C 30s, 72°C 1 min |
XRCC3 | (F) GCC TGG TGG TCA TCG ACT C (R) CAG GGC TCT GGA AGG CAC TGC TCA GCT CAC GCA cC | 136 | 30 cycles: 94°C 30s, 65°C 30s, 72°C 1 min |