2-ME-induced apoptosis in control and Shb-overexpressing (clone 14) PC3 cells. Cells were treated or not with 10 μM of 2-ME in the absence or presence of a p38 (SB203580) or c-Abl (STI-571) inhibitor. The inhibitor was added to the media 1–9 h before initiation of 2-ME treatment. Cells were treated for 12 h, before fixation and staining with DAPI and M30 for assessing apoptosis. The experiments were carried out in duplicates in 3 separate experiments, where 200–600 cells from each treatment condition was counted at 40 × magnification in a Zeiss immuno-fluorescence microscope. Means ± SEM are shown.