Figure 4

STAT3 decoy ODN decreased the expression of STAT3-regulated cell growth or anti-apoptotic genes. (A) After transfected with 25 nmol/L STAT3 decoy ODN or scramble ODN for 24 h, the whole A549 cells were harvested and the total cellular RNA was isolated using Trizol Reagent, mRNA levels of STAT3-correlated genes such as mcl-1, cyclin D1, bcl-xl, cyclinE, c-myc and survivin were detected using RT-PCR method. The PCR products were electrophoresed and photographed using AlphaEaseFC. (B) After transfected with 25 nmol/L decoy ODN, the whole A549 were harvested and the whole-cell extracts were obtained. And western blotting for bcl-x1 and cyclin D1 were then examined as described in materials and methods. Histogram presented the relative expression level of each gene or protein after normalization to its corresponding internal control. Statistical significance was determined as *p < 0.01 and ^# p < 0.05 compared to control.