A: Characteristics of C4-2 tumor-bearing tibiae. Upper panel: radiographs of the right and left tibiae of representative animals from control, prevention, and treatment groups. Arrows indicate areas of the eroded cortical shaft in tumored tibiae of C4-2-treated animals. Middle panel: mineralized tibiae were harvested 8 weeks after tumor injection and embedded in methacrylate and 6 μm sections were stained with Goldner's stain. In tumor-bearing animals the tumor replaced the marrow, filling the cavity and degrading the trabecular bone. Arrows indicate areas of the eroded cortical shaft where the tumor has started to extravasate. B: Bone mineral density (BMD) was measured 8 weeks after intra-tibial C4-2 injection in SCID mice. There was no significant difference in BMD between control and prevention (p = 0.1491) or control and treatment (p = 0.8601) animals. Results are presented as mean ± SE.