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Table 2 Detection of PAI-1 in cell-free supernatants.

From: Induction of plasminogen activator inhibitor type-1 (PAI-1) by hypoxia and irradiation in human head and neck carcinoma cell lines

  PAI-1 concentration [ng/ml]
Incubation time [d] BHY FaDu
0 66.3 (± 5.9) 9.8 (± 1.9)
1 66.8 (± 6.4) 10.5 (± 2.8)
2 61.0 (± 5.5) 10.2 (± 1.8)
3 66.9 (± 4.6) 9.8 (± 2.0)
7 73.3 (± 4.3) 10.4 (± 1.8)
  1. Cells were maintained for 24 h under hypoxic conditions, then the cell-free supernatants were incubated at 37°C under standard conditions for up to 7 days. PAI-1 concentrations [ng/ml] ± S.E.M. of three independent experiments, each analyzed in duplicate by ELISA are shown.