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Table 2 qPCR conditions

From: Association of oestrogen receptor beta 2 (ERβ2/ERβcx) with outcome of adjuvant endocrine treatment for primary breast cancer – a retrospective study

Gene

Primers/Probes3

Conc. (μM)

Amplicon

Cycling conditions

ERβ2 1

For-ATCCATGCGCCTGCTAAC

0.5

bases 1265–1343

50 cycles:

 

Rev-GAGTGTTTGAGAGGCCTTTTCTG

0.5

GenBank: AF124790

95°C 20s, 52°C 20s

 

Probe-TCCTGATGCTCCTGTCCCACGTCA

0.2

  

ERα 2

For-CCACCAACCAGTGCACCATT

1.0

bases 1030–1137

40 cycles: 95°C 20s

 

Rev-GGTCTTTTCGTATCCCACCTTTC

1.0

GenBank: NM_000125

60°C 20s, 72°C 30s

HPRT 2

For-GTGTTGGATATAAGCCAGACTTTGTT

1.0

bases 597–763

40 cycles:

 

Rev-AACTCAACTTGAACTCTCATCTTAGGC

1.0

GenBank: NM_000194

94°C 30s, 64°C 60s

GAPDH 2

For-GCATCCTGGGCTACACTGAG

0.5

bases 917–1079

40 cycles:

 

Rev-TCCACCACCCTGTTGCTGTA

0.5

GenBank: NM_002046

94°C 30s, 65°C 90s

  1. 1 primers from Critchley et al. [25];
  2. 2 primers designed in-house.
  3. 3 For = forward primer; Rev = reverse primer; Probe = Taqman probe.
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BMC Cancer

ISSN: 1471-2407

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