Table 1 Molecular Profiles of MCF10AT1 sublines (Control, E2, Tam and β-HCH selected). The markers shown based on mRNA expression of genes determined though quantitative RT-PCR. Values represent fold-increase of a marker's expression versus β-actin of a selected subline when compared to control (ethanol vehicle). N = 5–6 for each experiment * p < 0.05 and **p < 0.01 vs. treatment versus control.
From: Promotion of breast cancer by β-Hexachlorocyclohexane in MCF10AT1 cells and MMTV-neu mice
MCF10AT1 sublines selected for 20 passages in DMEM/F12 media containing: | ||||
|---|---|---|---|---|
Control | E2 (1 nM) | Tam (1 nM) | β-HCH (1 uM) | |
Proto-oncogenes | ||||
cNeu | 1.00 | 1.64* | 1.79* | 2.73** |
Cyclin D1 | 1.00 | 1.84* | 1.88* | 2.50** |
p21 | 1.00 | 2.05* | 1.07 | 1.19 |
p27 | 1.00 | 2.02* | 1.73* | 2.80* |
Cell Status | ||||
VEGF | 1.00 | 3.12* | 0.72 | 1.34 |
IGF-IR | 1.00 | 1.99* | 2.79* | 3.77** |
c-met | 1.00 | 2.06** | 0.66* | 1.26 |
CK19 | 1.00 | 0.78* | 0.72* | 1.67* |
Invasive | ||||
MMP-12 | 1.00 | 0.85 | 0.41* | 3.50* |
MMP-13 | 1.00 | 0.86 | 18.51** | 14.12** |
Inflammation | ||||
NFkB | 1.00 | 3.20** | 0.24* | 2.59** |