Figure 5
From: Overexpressed TP73 induces apoptosis in medulloblastoma
Genotoxic agents induce TP73 expression and apoptosis in medulloblastoma cell lines. (A) Chemotherapeutic agents (cisplatin (CDDP) 1 µM, etoposide (VP-16) 10 µM, and doxorubicin (DOXO) 1 µM) induce TP73 expression in Daoy cells, especially ΔN’p73 species, as determined by qRT-RTPCR normalized to GAPDH expression. (B) CDDP-treated (1, 5, and 25 µM) D283 cells display induction of TP53 and TP73 RNA expression (including ΔN’p73 species) - similar to results observed in CDDP-treated Daoy cells. Western immunoblots reveal that (C) Daoy cells and (D) D283 cells transiently transfected with p53-GFP expression plasmid increase expression of tagged p53-GFP protein (at a higher apparent molecular weight than native p53), as well as the p53/p73 target, p21Waf1 (arrows, upper panels). Treatment with etoposide (VP-16, 1.5 µM) stabilizes wild-type p53 protein. Transient transfection with either TAp73 or ΔNp73 expression plasmids also increased respective protein levels, normalized to ß-actin loading control. (arrows lower panels).