Figure 2

Primary medulloblastoma specimens and medulloblastoma cell lines overexpress TAp73 and ΔNp73 proteins. Western immunoblot analysis of p53, TAp73, and ΔNp73 protein expression in: (A) established medulloblastoma cell lines, Daoy and D283MED (D283), and (B) primary medulloblastoma specimens. Immunoblots for p53 (FL-393) or p73 proteins (H-79, GC-15, and IMG-313) were stripped and re-probed for β-actin (C-2) to control for protein loading. Columns marked with numbers 1–8 represent Western blots of eight different primary medulloblastoma specimens. Additional bands presumably represent additional carboxy-terminal isoforms of TAp73 and ΔNp73. Relative migration of molecular weight standards is shown at left. Shown are representative blots from at least 3 experiments, normalized to ß-actin (mean ± S.E.M.). (C) Primary medulloblastoma (MB) specimens and medulloblastoma cell lines display significant expression of TAp73, ΔNp73, and p53 proteins as quantitated on Western blots. Shown are representative blots from at least 3 experiments, normalized to ß-actin (mean ± S.E.M.).