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Figure 2 | BMC Cancer

Figure 2

From: Down-regulation of SFRP1 as a putative tumor suppressor gene can contribute to human hepatocellular carcinoma

Figure 2

The overexpression of SFRP1 can significantly inhibit the cell growth and colony formation of HCC cells. (A) Plasmid pcDNA3.0-SFRP1 was transiently transfected into YY-8103 cells, confirmed by immunoblotting assay, where empty vector was used as control and β-actin was used as an internal reference. (B) Cell growth curve of YY-8103 cells with the exogenous SFRP1, which cultured in RPMI 1640 with 10% FBS. The cells transfected with the empty vector pcDNA3.0 were served as control. The experiments were repeated at least three times. The result represents the average value of triplicate wells, with standard deviation. T-test was performed to determine the statistical significance between both vector and SFRP1 experiments using SPSS software, and p<0.05. (C) Plasmid pcDNA3.0-SFRP1 was also transiently transfected into Hep3B cells, where the overexpression of SFRP1 was confirmed by immunoblotting assay, as compared to the control transfected by empty vector. (D) The colony formation of Hep3B cells was markedly inhibited as transfected with exogenous SFRP1, where the empty vector pcDNA3.0 was served as control (left). Here, after transfection for 24 h, the cells were striped and plated on 100 mm-dishes and then cultured by G418 (600 mg/ml) for 3 weeks. The dishes were stained with crystal violet solution and the number of colonies was counted from three independent experiments. The right histogram showed the colony formation efficiency, where the numbers represented the average value of three independent experiments, with standard deviation (p < 0.01, as compared with that of vector control).

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