Comparative phenotypic analysis of Standard DC and mature FastDCs by flow cytometry. Surface expression of a panel of antigens was determined following maturation with TNFα, IL1β, IL6 and PgE2 for 24 hours (mature FastDCs) and 48 hours (Standard DC). Open diagram shows reactivity with control mAbs. Closed diagrams shows reactivity with anti HLA-DR and CD antibodies. The setting of the gates based on the forward (FSC) and site scatter (SSC) is indicated to the left in the figure. One representative of three performed experiments is presented.