| | CEA | CK20 | CK7 | Ki 67 |
---|
PMP-1 | Primary tumor | 100% m/c | 100% m/c | 0 | 10–50% |
| Main surgical specimen | 100% m/c | 100% m/c | 0 | 10–50% |
| p0 | 100% m/c | 100% m/c | <10% m | 10–50% |
| p1 | 100% m/c | 100% m/c | 0 | >50% |
| p2 | 100% m/c | 100% m/c | 0 | 10–50% |
| p3 | 100% m/c | 100% m/c | 0 | 10–50% |
| p4 | 100% m/c | 100% m/c | 0 | 10–50% |
| p5 | 100% m/c | 100% m/c | 0 | 10–50% |
| p6 | 100% m/c | 100% m/c | <10% m | 10–50% |
PMP-2 | Primary tumor | >50% m/c | 100% m/c | 10–50% m/c | <10% |
| Main surgical specimen | 100% m/c | 100% m/c | 100% m/c | 10–50% |
| p0 | 100% m/c | 100% m/c | 100% m/c | >50% |
| p1 | 100% m/c | 100% m/c | 100% m | >50% |
| p2 | 100%m/c | 100% m/c | 100% m | >50% |
| p3 | 100% m/c | 100% m/c | 100% m/c | >50% |
| p4 | 100% m/c | 100% m/c | >50% m | 10–50% |
| p5 | 100% m/c | 100% m/c | 100% m | >50% |
| p6 | 100% m/c | 100% m/c | 100% m | >50% |
- Numbers indicate percentage of cells stained in the section.
- m = membranous staining c = cytoplasmic staining
- A consistently high expression was seen for CEA and CK20 in both tumors as well as in tumor tissue harvested from the animals. The PMP-1 model was essentially negative for CK7 expression, with the exception of slight membranous staining in two of the passages, whereas PMP-2 exhibited a relatively consistent, high expression of this cytokeratin. A Ki67 positive cell fraction of 10–50% in the primary tumor was conserved throughout the animal passages in the PMP-1 animal model. In the PMP-2 model, low and intermediate Ki67 positive cell fractions were found in the primary tumor and main surgical specimen, respectively, whereas in tissue harvested from the animals the positive fraction was consistently high (>50%).