Table 1 Primary antibodies and immunostaining conditions used for immunohistochemical studies in primary tumors, main surgical specimens and xenografts.
Antibodies | Catalogue | Pretreatment | Dilution | Positive controls |
|---|---|---|---|---|
CEA | Dako M7072 | Low pH | 1/100 | Intestinal mucosa Colon cancer |
CK20 | Progen 61026 | Tris-EDTA | 1/100 | Intestinal mucosa Colon cancer |
CK7 | Dako M7018 | Tris-EDTA | 1/200 | Intestinal mucosa Colon cancer |
Ki67 | Dako M7240 | Tris-EDTA | 1/150 | Appendix |