Figure 8

Isolation and characterization of LECs from lymphangiomas. Lymphendothelial cells (LECs) from lymphangioma explants isolated by „cell sweeping” were photographed under phase contrast microscopy. a) The cells from patient-A show the typical cobblestone morphology and are contact inhibited (passage 7, magnification, × 40.). Expression of CD31/PECAM-1 together with Prox1 in LECs (b). Membranous localization of CD31 and nuclear localization of Prox1 was detected by immunocytology. Magnification, × 200. c) Same specimen as in b) showing CD31 expression and nuclear counter-staining with DAPI. Magnification, × 200. d) Podoplanin staining. Nuclei were counter-stained with DAPI. One representative staining out of several independent experiments is shown.