Transcription profiles of non-immortalized breast cancer cell lines

BMC Cancer

Table 1 Cell origin and RNA expression of some frequently used markers

cell line	origin ^(a)	KT14	KT18	Vim	ER	ERBB2	ERBB3	MGB1	MGB2
HMEC #1	mammary epithelial	+	+	++	+/-	+	+	+/-	+
HMEC #2	mammary epithelial	+	+	+	ND	ND	ND	ND	ND
HMEC #3	mammary epithelial	ND	ND	ND	-	+	+	-	+
HMEC #6	mammary epithelial	+	+	++	-	+	+	-	+
HMEC #7	mammary epithelial	+	+	++	+/-	+	+	-	+
HMEC #8	mammary epithelial	ND	ND	ND	+/-	+	+	+	+
HMEC #9	mammary epithelial	+	+	++	+	+	+	+	+
HMEC #10	mammary epithelial	+	+	++	+/-	+	+	+	+
HMEC #11	mammary epithelial	+	+	++	+	+	+	-	+
HMEC #12	mammary epithelial	+	+	+	+	+	+	-	+
HMEC #13	mammary epithelial	+	+	+	-	+	+	-	+
MSSM 3	ascites	-	+	++	+	+	+/-	-	-
MSSM 4	pleural effusion	-	+	++	-	+	-	-	+
MSSM 5	pleural effusion	-	+	++	+	+	+/-	-	+
MSSM 6	pleural effusion	-	+	++	+/-	+	+	-	+
MSSM 7	pleural effusion	+	+	++	-	+	-	+	-
MSSM 8	pleural effusion	-	+	++	+/-	+	+	+	+
MSSM 9	ascites	-	+	++	+/-	+	-	+	+
MSSM 10	pleural effusion	-	+	++	-	+	+/-	+	+
MSSM 11	pleural effusion	-	+	++	+/-	+	+	+	+
MSSM 14	ascites	-	+	++	+/-	+	-	+	+
MTSV1-7	from milk	-	+	++	-	+	+/-	-	-
MCF 10A	mammary gland, adherent	+	+	++	+/-	+	+	+	+
MCF 10F	mammary gland, floating	+	+	+/-	+	+	+	-	+
MDA-MB 231	pleural effusion	-	+	++	-	+	+	-	+
MDA-MB 453	effusion	-	+	+	-	+ ^(c)	+	+	+
MDA-MB 468	pleural effusion	+	+	+	-	+	+	+	-
MCF 7 N	pleural effusion	-	+	+/-	+	+	+	-	+
MCF 7 P	pleural effusion	-	+	+	+	+	+	+	+
T47D	pleural effusion	-	+	-	+	+	+	+	+
BT20	prim tumor	+	+	+/-	+ ^(b)	+	+	+	+
BT474	invasive ductal carc	ND	ND	ND	-	+	ND	+	+

Determination of the presence of mRNA for the KT and VIM markers shows the results of the array experiment. Determination for the receptors was done in triplicate by RT-PCR. +/-: 1/3 show the specific product of the PCR, -: none of the three, +: all of them, ND: not done.
(a) HMECs and established cell lines according to the providers (Clonetics, ATCC).
(b) It is known that BT20 transcribes an mRNA for this gene with a deletion in the fifth exon and hence the cell does not express the protein (ATCC).
(c) MDA-MB 453 showed a 27.2 fold up-regulation when compared to the HMECs (29.3 with MCF 10A), consistent with its amplified ERBB2 gene.

ISSN: 1471-2407